Method development and validation for the estimation of carboplatin in pharmaceutical pure and dosage form using UV-Vis method

Abstract

High incidences and cancer cases in the world for the past decade have resulted in the need to conduct extensive research on the cancer medications. Carboplatin is a platinum compound used mainly in the treatment of lung, head and neck, as well as many other cancers. No method has been reportedly done and developed for the routine analysis of carboplatin using UV-Vis. Many have reported HPLC and Mass spectroscopy. A method was developed and validated for the analysis of carboplatin in the standard and injection form. 10mg of the carboplatin standard was transferred into a 10ml volumetric flask, and diluted up to the mark using 0.1M NaOH. The solution was further diluted to a 1µg/ml solution and scanned using UV-Vis. The ʎ-max was found to be 295nm. A calibration curve was plotted and at 2-10µg/ml Beer’s law was obeyed. The Linear equation was y=0.0564x+0.0057. The r2 value was 0.9996, which was within acceptable limits. Precision using inter-day and intra-day precision were done yielding %RSD values of 0.734 and 0.504 respectively, which were acceptable according to ICH guidelines (≤2). A method was therefore successfully developed and validated for routine use in the analysis of Carboplatin in the standard and parenteral dosage form.